General characteristics of Lymnaea stagnalis (Gastropoda, Lymnaeidae) hemolymph cells population composition
DOI:
https://doi.org/10.33910/2686-9519-2026-18-1-30-40Keywords:
Lymnaea stagnalis, pulmonary mollusks, hemocytes, hemolymph, flow cytometry, cell populations, LysoTracker, SYTO62, MitoTrackerAbstract
The cellular composition of the hemolymph of the pulmonary mollusk Lymnaea stagnalis has been studied. Based on the results of light microscopy, scanning electron microscopy, and flow cytometry, two functionally active cell populations (hyalinocytes and granulocytes) were identified in the hemolymph. These cells differ in morphology, complexity of the cytoplasmic compartment, and in their ability to form pseudopodia. The use of specific fluorescent dyes and a sequential gating strategy based on light scattering and fluorescence parameters made it possible to determine the subpopulation composition of circulating hemocytes characterized by different levels of metabolic activity.
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Copyright (c) 2026 Андрей Алексеевич Комиссаров , Мария Константиновна Серебрякова, Станислав Викторович Сенкевич, Арина Сергеевна Токмакова
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