Cellular composition of the hemolymph of Viviparus viviparus molluscs (Gastropoda: Prosobranchia)
DOI:
https://doi.org/10.33910/2686-9519-2024-16-2-284-293Keywords:
Viviparus viviparus, hemocytes, flow cytometry, hemocyte viabilityAbstract
The cellular composition of the hemolymph of the prosobranch mollusc Viviparus viviparus was studied. Based on the results of morphometric and cytofluorimetric analysis, two functionally active populations of cells were identified in the hemolymph, which differ in cells’ size, as well as in the ability to form pseudopodia and the complexity of the cytoplasmic compartment. In addition to the main ones, a transitional population was identified, which is represented by cells at different stages of maturation and specialization. It has been suggested that prosobranch V. viviparus have one line of differentiation of hemolymph cells.
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Ataev, G. L., Prokhorova, E. E., Kudryavtsev, I. V., Polevshchikov, A. V. (2016) The influence of trematode infection on the hemocyte composition in Planorbarius corneus (Gastropoda, Pulmonata). // Invertebrate survival journal, vol. 13, pp. 164–171. DOI: https://doi.org/10.25431/1824-307X/isj.v13i1.164-171
Ataev, G. L., Prokhorova, Е. Е., Tokmakova, А. S. (2020) Zashchitnye reakcii legochnyh mollyuskov pri parazitarnoj invazii [Defense reactions of pulmonate molluscs during parasitic invasion]. Parazitologiya – vol. 54, no. 5, pp. 371–401. DOI: https://doi.org/10.31857/S1234567806050028
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Gorbushin, A. M., Iakovleva, N. V. (2006) Haemogram of Littorina littorea. Journal of the Marine Biological Association of the United Kingdom, vol. 86, no. 5, pp. 1175–1181. DOI: https://doi.org/10.1017/S0025315406014160
Ottaviani, E. (1989) Haemocytes of the freshwater snail Viviparus ater (Gastropoda, Prosobranchia). Journal of molluscan studies, vol. 55, no. 3, pp. 379–382. DOI: https://doi.org/10.1093/mollus/55.3.379
Ottaviani, E., Franchini, A., Fontanili, P. (1992) The presence of immunoreactive vertebrate bioactive peptide substances in hemocytes of the freshwater snail Viviparus ater (Gastropoda, Prosobranchia). Cellular and Molecular Neurobiology, vol. 12, no. 5, pp. 455–462. DOI: https://doi.org/10.1007/BF00711546
Pila, E. A., Sullivan, J. T., Wu, X. Z. et al. (2016) Haematopoiesis in molluscs: a review of haemocyte development and function in gastropods, cephalopods and bivalves. Developmental and Comparative Immunology, vol. 58, pp. 119–128. DOI: https://doi.org/10.1016/j.dci.2015.11.010
Prokhorova, Е. Е., Serebryakova, М. S., Tokmakova, А. S. et al. (2018) The analysis of cell composition in the hemolymph of three Planorbidae species (Gastropoda: Pulmonata). Invertebrate Zoology, vol.15, no.1, pp. 103–113. DOI: https://doi.org/10.15298/invertzool.15.1.08
Ray, M., Bhunia, N. S., Bhunia, A. S., Ray, S. (2013) A comparative analyses of morphological variations, phagocytosis and generation of cytotoxic agents in flow cytometrically isolated hemocytes of Indian molluscs. Fish and shellfish immunology, vol. 34, no. 1, pp. 244–253. DOI: https://doi.org/10.1016/j.fsi.2012.11.006
Serebryakova, M. K., Tokmakova, A. S., Prokhorova, E. E., Ataev, G. L. (2022) Changes in the cell composition of the hemolymph in the snail Planorbarius corneus after infection with the trematode Plagiorchis sp. Invertebrate biology, vol. 141, no. 4, e12389. DOI: https://doi.org/10.1111/ivb.12389
Suwannatrai, K., Suwannatrai, A. T., Donthaisong, C. et al. (2019) Hemocyte subpopulation changes in Bithynia snails infected with Opisthorchis viverrini in Thailand. bioRxiv, pp. 536292. DOI: https://doi.org/10.1101/536292
Suwannatrai, K. Suwannatrai, A. T., Loukas A., Sotillo J. (2020) Recent advances on the immunobiology of Bithynia spp. hosts of Opisthorchis viverrini. Developmental and Comparative Immunology, vol. 102, 103460. DOI: https://doi.org/10.1016/j.dci.2019.103460
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