A new species of the genus Brachytarsophrys Tian & Hu, 1983 (Anura: Megophryidae) from Guangxi, China
DOI:
https://doi.org/10.33910/2686-9519-2026-18-2-586-598Keywords:
Brachytarsophrys, Jinxiu County, mitochondrial, morphology, new species, phylogeny, Short-Legged Toad, taxonomyAbstract
A new species of the genus Brachytarsophrys is described from Guangxi Zhuang Autonomous Region, China. The new species can be distinguished from its congeners by a combination of morphological characters: body size relatively small, dorsal skin smooth with a few large tubercles on dorsum and crotch, snout relatively long, eye relatively small, foot relatively short, pectoral glands orange, upper iris blood red and lower iris reddish brown, nuptial pad present on first finger in adult male. Phylogenetically, the new species differs from other species of the genus by a genetic divergence of 4.2–15.7% and 3.3–15.2% in COI and cytb genes, respectively. This study brings the total number of the species of Brachytarsophrys to 12, with ten occur in China and three in Guangxi.
References
References
AmphibiaChina. (2026) The database of Chinese amphibians. [Online]. Available at: http://www.amphibiachina.org/ (accessed 13.01.2026). (In English)
Che, J., Chen, H.-M., Yang, J.-X. et al. (2012) Universal COI primers for DNA barcoding amphibians. Molecular Ecology Resources, vol. 12, no. 3, pp. 247–258. https://doi.org/10.1111/j.1755-0998.2011.03090.x (In English)
Edgar, R. C. (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, vol. 32, no. 5, pp. 1792–1797. https://doi.org/10.1093/nar/gkh340 (In English)
Frost, D. R. (2026) Amphibian species of the world. Version 6.2. American Museum of Natural History. Available at: https://amphibiansoftheworld.amnh.org/index.php (accessed 13.01.2026). (In English)
He, Y., Ai, R., Huang, J. et al. (2024) Morphological and molecular data revealed one new species of the short-legged toads Brachytarsophrys Tian and Hu, 1983 (Anura, Megophryidae) from Yunnan, China. Asian Herpetological Research, vol. 15, no. 1, pp. 22–30. https://doi.org/10.3724/ahr.2095-0357.2023.0042 (In English)
Kalyaanamoorthy, S., Minh, B. Q., Wong, T. K. F. et al. (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods, vol. 14, no. 6, pp. 587–589. https://doi.org/10.1038/nmeth.4285 (In English)
Li, Y., Zhang, D.-D., Lyu, Z.-T. et al. (2020) Review of the genus Brachytarsophrys (Anura: Megophryidae), with revalidation of Brachytarsophrys platyparietus and description of a new species from China. Zoological Research, vol. 41, no. 2, pp. 105–122. https://doi.org/10.24272/j.issn.2095-8137.2020.033 (In English)
Lyu, J.-C., Zhou, S.-L., Zhang, T.-B. et al. (2026) Morphological and molecular data reveal one new species of the genus Brachytarsophrys Tian & Hu, 1983 (Anura, Megophryidae) from Guizhou, China. Zoosystematics and Evolution, vol. 102, no. 1, pp. 71–85. https://doi.org/10.3897/zse.102.161437 (In English)
Myers, C. W., Duellman, W. E. (1982) A new species of Hyla from Cerro Colorado, and other tree frog records and geographical notes from western Panama. American Museum Novitates, no. 2752, pp. 1–32. (In English)
Nguyen, L.-T., Schmidt, H. A., von Haeseler, A., Minh, B. Q. (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating maximum-likelihood phylogenies. Molecular Biology and Evolution, vol. 32, no. 1, pp. 268–274. https://doi.org/10.1093/molbev/msu300 (In English)
Ronquist, F., Teslenko, M., van der Mark, P. et al. (2012) MrBayes 3.2: Efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, vol. 61, no. 3, pp. 539−542. https://doi.org/10.1093/sysbio/sys029 (In English)
Tamura, K., Stecher, G., Kumar, S. (2021) MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution, vol. 38, no. 7, pp. 3022–3027. https://doi.org/10.1093/molbev/msab120 (In English)
Wu, X., Pan, Y., Xiao, W. et al. (2025) Integrative taxonomic evidence for a new species of the short-legged toads Brachytarsophrys (Anura, Megophryidae) from Guangxi, China. Zoosystematics and Evolution, vol. 101, no. 4, pp. 1369–1382. https://doi.org/10.3897/zse.101.157834 (In English)
Zhang, P., Liang, D., Mao, R.-L. et al. (2013) Efficient sequencing of Anuran mtDNAs and a mitogenomic exploration of the phylogeny and evolution of frogs. Molecular Biology and Evolution, vol. 30, no. 8, pp. 1899–1915. https://doi.org/10.1093/molbev/mst091 (In English)
Zhao, J., Yang, J., Chen, G. et al. (2014) Description of a new species of the genus Brachytarsophrys Tian & Hu, 1983 (Amphibia: Anura: Megophryidae) from southern China based on molecular and morphological data. Asian Herpetological Research, vol. 5, no. 3, pp. 150–160. https://doi.org/10.3724/SP.J.1245.2014.00150 (In English)
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